Journal: Frontiers in Immunology
Article Title: Integrated single-cell and bulk RNA sequencing analysis reveals ACACA as a potential prognostic and immunotherapeutic biomarker across cancers
doi: 10.3389/fimmu.2025.1599223
Figure Lengend Snippet: Validation of ACACA expression levels in clinical tissue samples and cell lines. (A) Representative images of ACC1 staining in LUAD tumor tissue and paracancerous tissue from clinical samples. Scale bar: 50 mm. (B) Analysis of ACC1 expression in tumor tissues and normal tissues. (C) The Kaplan-Meier’s survival curve according to ACC1 expression levels. (D) Subcellular localization and expression intensity of ACC1 in HCC827 and PC9 cells. (E) The relative expression of ACC1 in LUAD cell lines (HCC827, PC9, A549, and H1299) examined by western blot; human bronchial epithelial (BEAS-2B) cell as control. The symbols indicate the level of significance: *** for p < 0.001.
Article Snippet: These paraffin-embedded samples were stained with anti-ACC1 antibody (1:200 dilution; Cell Signaling Technology, #3676).
Techniques: Biomarker Discovery, Expressing, Staining, Western Blot, Control